Experimental oncology
Prof. Dr hab. Michał Mikula
3 months
Visiting Observership
English
Programme description
This intensive 12-week laboratory program offers a comprehensive introduction to the core techniques and analytical methods used in molecular and translational cancer research. Structured across three thematic months, participant will progress from mastering foundation cell culture and nucleic acid techniques to applying advanced methods in genomics, proteomics, and in vivo models.
Participatnt will gain hands-on experience in culturing human cancer cell lines, isolating nucleic acids, performing qPCR, transfection of cancer cells and methylation assays. The course further includes extensive training in protein isolation, mass spectrometry, and Western blotting, as well as molecular cloning and bacterial expression systems.
The final module introduces metabolomics (via GC-MS and Seahorse assays) and practival animal experimentation under FELASA guidelines, providing critical link between molecular methods and translational applications.
By the end of the program, trainees will have acquired a robust technical skill set essential for preclinical cancer research and be well-prepared for academic or industry-based biomedical research roles.